In 2016, The New York Botanical Garden began the New York City EcoFlora project. An EcoFlora combines established knowledge from herbarium specimens and the scientific literature with real-time observation of plants and interactions, and thus contributes not only to our body of scientific data, but also provides opportunities for education and informs conservation. Monthly EcoQuest Challenges encourage volunteer citizen scientists to document the native and naturalized flora of New York City by taking and sharing photos via iNaturalist. Since the first EcoQuest Challenge in August 2017, 832 citizen scientists have recorded over 55,000 observations across all five boroughs of New York City, documenting the native flora, invasive species, and biotic interactions among plants, animals, and fungi. Participants will be able to explore the possibilities of creating an EcoFlora project at their own institution.